Product ID:ETHIDIUM BROMIDE, 331565
MSDS Date:03/02/1999
FSC:6505
NIIN:00N092485
Status Code:A
MSDS Number: CJTCX

Emergency Phone: 800-424-9300 (CHEMTREC)

Info Phone: 800-854-3417

Cage: 29089

Telephone: 619-450-5558; 800-854-3417

Cage: 29089

ETHIDIUM BROMIDE

CAS: 1239-45-8

RTECS: SF7950000

OSHA PEL N/K

ACGIH TLV: N/K

LD50 LC50 Mixture:SEE TOXICOLOGICAL INFORMATION.
Routes of Entry: Inhalation:YES Skin:YES Ingestion:YES
Reports of Carcinogenicity:NTP:NO IARC:NO OSHA:NO
Health Hazards Acute and Chronic:ACUTE: MAY BE HARMFUL BY INGESTION,
INHALATION OR ABSORPTION THROUGH SKIN. MATERIAL MAY CAUSE
IRRITATION TO THE EYES, SKIN, MUCOUS MEMBRANES, AND UPPER
RESPIRATORY TRACT. CHRONIC: THERE IS LIMITED EVI DENCE THAT
ETHIDIUM BROMIDE IS MUTAGENIC IN LABORATORY CELL CULTURES. UNTIL
FURTHER TESTING HAS BEEN DONE, IT SHOULD BE TREATED AS A POSSIBLE
MUTAGEN IN HUMANS.
Effects of Overexposure:SEE HEALTH HAZARDS.

First Aid:SKIN: WASH IMMEDIATELY WITH SOAP AND COPIOUS AMOUNTS OF WATER
FOR AT LEAST 15 MINUTES. REMOVE CONTAMINATED CLOTHING AND SHOES AND
WASH BEFORE WEARING. CONSULT A PHYSICIAN. EYES: FLUSH IMMEDIATELY
WITH COPIOUS AMOUNTS OF WATER FOR AT LEAST 15 MINUTES. ASSURE
ADEQUATE FLUSHING BY SEPARATING THE EYELIDS WITH FINGERS. CONSULT A
PHYSICIAN. INHALATION: REMOVE TO FRESH AIR AND MONITOR BREATHING.
IF BREAT HING BECOMES DIFFICULT, GIVE OXYGEN AND CONSULT A
PHYSICIAN. IF BREATHING STOPS, GIVE ARTIFICIAL RESPIRATION AND
CONSULT A PHYSICIAN. INGESTION: WASH MOUTH OUT WITH WATER AND
CONSULT THE LOCAL POISON CENTER AND A PHYSICIAN.

Extinguishing Media:DRY CHEMICAL POWDER, CARBON DIOXIDE, WATER SPRAY,
ALCOHOL OR POLYMER FOAM.
Fire Fighting Procedures:WEAR NIOSH APPROVED SCBA AND FULL PROTECTIVE
EQUIPMENT .
Unusual Fire/Explosion Hazard:UPON THERMAL DECOMPOSITION, IT MAY EMIT
TOXIC GASES.

Spill Release Procedures:EVACUATE AREA. WEAR A NIOSH APPROVED
RESPIRATOR, CHEMICAL SAFETY GOGGLES, RUBBER BOOTS, AND HEAVY RUBBER
GLOVES. SWEEP UP AND PLACE IN A SUITABLE CONTAINER. AVOID RAISING
DUST. HOLD FOR APPROPRIATE DI SPOSAL. WASH SPILL SITE AND VENTILATE
AREA AFTER MATERIAL PICKUP IS COMPLETE.

Handling and Storage Precautions:STORE IN A TIGHTLY CLOSED CONTAINER IN
A COOL, DRY PLACE (+20C). PROTECT FROM LIGHT. MATERIAL IS AN
IRRITANT. POSSIBLE MUTAGEN. AVOID CONTACT W/MATERIAL. AVOID
PROLONGED/REPEATED EXPOSURE. LAB SHOULD BE EQUIPPED WITH A SAFETY
SHOWER & AN EYE WASH STATION. WASH THOROUGHLY AFTER HANDLING
MATERIAL.
Other Precautions:MATERIAL SHOULD ONLY BE HANDLED BY QUALIFIED,
EXPERIENCED PROFESSIONALS.

Respiratory Protection:USE A NIOSH APPROVED RESPIRATOR.
Ventilation:HANDLE IN A CHEMICAL FUME HOOD WITH ADEQUATE VENTILATION.
Protective Gloves:CHEMICAL RESISTANT GLOVES.
Eye Protection:ANSI APPROVED CHEMICAL WORKERS GOGGLES .
Other Protective Equipment:ANSI APPROVED EMERGENCY EYEWASH AND DELUGE
SHOWER .
Work Hygienic Practices:WASH THOROUGHLY AFTER HANDLING.
Supplemental Safety and Health
SYNONYMS: ETHIDIUM BROMIDE; HOMODIUM BROMIDE. MOLECULAR WEIGHT: 394.3.
MOLECULAR FORMULA: C*21H*20N*3. BR.

Melt/Freeze Pt:>260.C, 500.F
M.P/F.P Text:260C - 262C
Solubility in Water:SOLUBLE
Appearance and Odor:PURPLE-RED SOLID.

Stability Indicator/Materials to Avoid:YES
AVOID CONTACT WITH STRONG OXIDIZING AGENTS.
Stability Condition to Avoid:STABLE UNDER NORMAL HANDLING PROCEDURES.
Hazardous Decomposition Products:UPON THERMAL DECOMPOSITION, MAY EMIT
TOXIC GASES INCLUDING CARBON MONOXIDE, CARBON DIOXIDE, NITROGEN
OXIDES, AND HYDROGEN BROMIDE GAS.

Waste Disposal Methods:COUNTRY, FEDERAL, STATE, & LOCAL REGULATIONS ARE
VARIED & CHANGE FREQUENTLY. FOR THIS REASON, MANUFACTURER
RECOMMENDS THAT YOU CONTACT YOUR LOCAL DEPARTMENT OF HEALTH
SERVICES FOR INFORMATION ON THE D ISPOSAL OF THIS PRODUCT/ARRANGE
FOR DISPOSAL BY A LICENSED DISPOSAL COMPANY. DISPOSE OF IN
ACCORDANC E WITH LOCAL, STATE, & FEDERAL REGULATIONS .

Toxicological Information:RTECS #: SF7950000. TOXICITY DATA &
REFERENCES: IPR; MUS; LDLO: 20 MG/KG ACTIVE AS ANTI-CANCER AGENT
1974. CANCER RESEARCH. 34, 2699. SCU; MUS; LD50: 110 MG/KG 1952.
ANNALS OF TROPICAL MEDICINE & PARA SITOLOGY. 46, 285. GENETIC DATA
& REFERENCES: LEU; HMN; DOSE: 5 MG/L DNA DMG 1976. NATURE. 263,
679. LVR; RAT; DOSE: 2500 NMOL/L UNSCHEDULED DNA SYNTHESIS 1989.
CARCINOGENESIS (LONDON). 10, 393. HLA; HMN; DOSE: 40 UMOL/L DNA
INHIBITION 1982. MUTATION RESEARCH. 92, 427. FBR; HMN; DOSE: 2
UMOL/L DNA ADDUCT 1975. CHEMICO-BIOLOGICAL INTERACTIONS. 11, 101.
LNG; HAM; DOSE: 75 UMOL/L MUTATIONS IN MAMMALI AN SOMATIC CELLS
1994. (ECOL INFO)

Disclaimer (provided with this information by the compiling agencies):
This information is formulated for use by elements of the Department
of Defense. The United States of America in no manner whatsoever,
expressly or implied, warrants this information to be accurate and
disclaims all liability for its use. Any person utilizing this
document should seek competent professional advice to verify and
assume responsibility for the suitability of this information to their
particular situation.

Ecological:NO INFORMATION AVAILABLE. --CARCINOGENESIS (LONDON). 15,
2303. FBR; MUS; DOSE: 1 MG/L CYTOGENETIC ANALYSIS 1975. MUTATION
RESEARCH. 33, 417. LVR; RAT; DOSE; 10 MG/L SISTER CHROMATID
EXCHANGE 1986. CEL L BIOLOGY & TOXICOLOGY. 2, 379. LYM; MU; DOSE:
100 UMOL/L OTHER MUTATION TEST SYS 1972. BIOCHEM PHARMACOLOGY. 21,
1977. REFER TO REGISTRY OF TOX EFTS OF CHEM SUBSTANCES (RTECS) FOR
DEFINITION OF ABBRE VIATIONS USED IN ABOVE TEXT & FOR ADDNL INFO.
THIS RPT CONTAINS ONLY SELECTED INFO FROM RTECS.

Transport Information:UN NUMBER: N/A. PROPER SHIPPING NAME: N/A. IATA
CLASS: N/A. IATA PACKING GROUP: N/A.

Federal Regulatory Information:XI. RISK PHRASES: R36/37/38: IRRITATING
TO EYES, RESPIRATORY SYSTEM AND SKIN. R46: MAY CAUSE HERITABLE
GENETIC DAMAGE. SAFETY PHRASES: S22: DO NOT BREATHE DUST. S24/25:
AVOID CONTACT WITH SKIN AND EYE S. S26: IN CASE OF CONTACT WITH
EYES, RINSE IMMEDIATELY WITH PLENTY OF WATER AND SEEK MEDICAL
ADVICE. S36/37/39: WEAR SUITABLE PROTECTIVE CLOTHING, GLOVES AND
EYE/FACE PROTECTION. S45: IN CASE OF ACCI DENT OR IF YOU FEEL
UNWELL, SEEK MEDICAL ADVICE IMMEDIATELY. S53: AVOID EXPOSURE -
OBTAIN SPECIAL IN STRUCTIONS BEFORE USE.
State Regulatory Information: